Introduction to Cell Culture Techniques for Regenerative Therapies
FEBRUARY 4 – 8, 2013 | Associate Laboratory IBMC.INEB, Rua Campo Alegre 823, 4150-180 Porto
This course, organised by INEB, will cover the principles of cell culture theory for tissue engineering and regenerative medicine, with strong emphasis in state-of-the-art techniques for cell-identification and cellular properties analysis. Focus will be on stem cells (pluripotent embryonic stem cells, and multipotent stem cells, namely mesenchymal stem/stromal cells), endothelial and immune (myeloid and lymphoid) cells.
FINAL PROGRAMME:
MONDAY, 4TH OF FEBRUARY
09.00 – 09.30 COURSE OVERVIEW (Auditorium B)
09.30 – 10.30 CELLS FOR TISSUE ENGINEERING AND REGENERATIVE MEDICINE (Auditorium B)
Perpétua Pinto-do-Ó, INEB
11.00 – 12.00 STEM CELLS AND THE STEM CELL NICHE CONCEPT (Auditorium B)
Perpétua Pinto-do-Ó, INEB
13.00 – 14.30 MESENCHYMAL STEM/STROMAL CELLS (Auditorium B)
Catarina Almeida, INEB
14.30 – 16.30 KEY LECTURE “ENDOTHELIAL CELLS” INFLAMMATORY CELLS (Auditorium B)
Raquel Soares, Facilty of Medicine of the University of Porto
16.30 – 18.00 INFLAMMATORY CELLS (Auditorium B)
Susana Santos, INEB
18.00 – 18.30 COURSE ASSIGNMENT (Auditorium B)
TUESDAY, 5TH OF FEBRUARY
09.00 – 10.30 BASICS OF CELL CULTURE I (Auditorium B)
Perpétua Pinto-do-Ó, Catarina Almeida, INEB
11.00 – 12.30 BASICS OF CELL CULTURE II (Auditorium B)
Ana Freire, INEB
14.00 – 15.30 KEY LECTURE "FROM A CELL PRODUCT TO A CELL-BASED THERAPY, THE INDUSTRY EXPERIENCE"
(Auditorium B)
Miguel Santos, ECBio
15.30 – 18.30 COURSE ASSIGNMENT "PROJECT PREPARATION" (Auditorium B)
WEDNESDAY, 6TH OF FEBRUARY
10.30 – 12.30 ADVANCED CELL CULTURE: 2D vs 3D (Auditorium B)
Cristina Barrias, Isabel Amaral, Sílvia Bidarra, Raquel Gonçalves, INEB
14.00 – 15.30 KEY LECTURE "THE PHYSICO-CHEMICAL MICROENVIRONMENT" (Main Auditorium)
Arti Ahluwalia, University of Pisa, Italy
16.00 – 17.30 KEY LECTURE “BIOREACTORS” (Main Auditorium)
Arti Ahluwalia, University of Pisa, Italy
THURSDAY, 7TH OF FEBRUARY
09.00 – 09.30 ELISA (Main Auditorium)
Fabíola Moutinho, INEB
09.30 – 10.30 IMMUNOCYTOCHEMISTRY (Main Auditorium)
Mariana Valente, INEB
11.00 – 12.30 FLOW CYTOMETRY AND FACS (Main Auditorium)
Catarina Leitão, IBMC
14.00 – 16.30 MICROSCOPY: CONFOCAL MICROSCOPY AND IMAGING FLOW CYTOMETRY (Main Auditorium)
Maria Lázaro, INEB
FRIDAY, 8TH OF FEBRUARY
09.00 – 12.30 COURSE ASSIGNMENT "PROJECT PREPARATION"
14.00 – 16.30 COURSE ASSIGNMENT "PROJECT PRESENTATION/ DISCUSSION" (Auditorium B)
INVITED SPEAKERS:
Prof. Arti Ahluwalia (University of Pisa, Italy); Prof. Raquel Soares (Faculdade de Medicina da Universidade do Porto, Portugal); Research Scientist from the Industry: Dr Miguel Santos (ECBio, R&D in Biotechnology, S.A.)
REGISTRATION:
The course will be open to up to 15 students until January 28. For registration please contact Gabriela.afonso@ineb.up.pt. A 100€ fee will cover support course material and lunch. A Certificate of Attendance is issued upon completion of the course.
CO-SPONSORING:
Marie Curie Initial Training Networks (ITN), FP7-PEOPLE-2012-ITN-EID IB2 “Industrial Biological Biomaterials Doctorate”

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